Phosphoproteomics

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Among the numerous types of post-translational protein modifications (PTMs), phosphorylation is a widespread class of modifications. Phosphorylated proteins account for approximately 1/3 to 2/3 of all eukaryotic proteins. Whereas, quantitative analysis of phosphorylation modification sites has been challenging because the extent of site-specific phosphorylation modifications may range from less than 1% to greater than 90% and is often low. Highly specific isolation enrichment methods can greatly improve the analysis depth of phosphorylated proteomes.

Common PTMs

The DIA technology combined with highly specific phosphoproteome isolation techniques realizes high fidelity and high depth protein phosphorylation modification profiling and analysis. 

The 4D-protein phosphorylation modification omics analysis which combines 4D-proteomics technology with the existing protein phosphorylation modification omics analysis can obtain protein phosphorylation modification profiling with higher accuracy compared with conventional techniques and offers technical support for related biological and medical research.

TMT-labeling proteomics and phosphoproteomics analysis

Mass Spectrometers:

timsTOF Pro;

Bruker Orbitrap Exploris™ 480

 

Application Scenarios:

For phosphorylation proteomics analysis

References:

1.Robust phosphoproteome enrichment using monodisperse microsphere-based immobilized titanium (IV) ion affinity chromatogra phy. Nature Protocols. 2013. 

2.Fishing the PTM proteome with chemical approaches using functional solid phases. Chemical Society Reviews. 2015.

Among the numerous types of post-translational protein modifications (PTMs), phosphorylation is a widespread class of modifications. Phosphorylated proteins account for approximately 1/3 to 2/3 of all eukaryotic proteins. Whereas, quantitative analysis of phosphorylation modification sites has been challenging because the extent of site-specific phosphorylation modifications may range from less than 1% to greater than 90% and is often low. Highly specific isolation enrichment methods can greatly improve the analysis depth of phosphorylated proteomes.

Common PTMs

The DIA technology combined with highly specific phosphoproteome isolation techniques realizes high fidelity and high depth protein phosphorylation modification profiling and analysis. 

The 4D-protein phosphorylation modification omics analysis which combines 4D-proteomics technology with the existing protein phosphorylation modification omics analysis can obtain protein phosphorylation modification profiling with higher accuracy compared with conventional techniques and offers technical support for related biological and medical research.

TMT-labeling proteomics and phosphoproteomics analysis

Mass Spectrometers:

timsTOF Pro;

Bruker Orbitrap Exploris™ 480

Application Scenarios:

For phosphorylation proteomics analysis

References:

  1. Robust phosphoproteome enrichment using monodisperse microsphere-based immobilized titanium (IV) ion affinity chromatogra phy. Nature Protocols. 2013. 
  2. Fishing the PTM proteome with chemical approaches using functional solid phases. Chemical Society Reviews. 2015.
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